Photoactive Subunits of Protochlorophyll(ide) Holochrome
نویسندگان
چکیده
منابع مشابه
Purification of protochlorophyllide holochrome.
Phototransformable protochlorophyllide holochrome was prepared from etiolated bean leaves. The detergent Triton X-100 in the presence of glycerol and tricine-KOH buffer (pH 8) enhanced the extractability, specific activity, and phototransformability of the holochrome. Purification was achieved by polyethylene glycol-6000 precipitation and hydroxyl-apatite, DEAE-cellulose, and agarose chromatogr...
متن کاملEnzymic nature of the protein moiety of protochlorophyllide holochrome.
The enzymic nature of the protein moiety of protochlorophyll(ide) holochrome was studied by following the fate of the [(14)C]protochlorophyll(ide) formed when dark-grown barley (Hordeum vulgare) or bean (Phaseolus vulgaris) leaves are incubated in the dark with 3 mm 4-delta-[(14)C]aminolevulinic acid. It was found that: [List: see text]Since turnover of protochlorophyll(ide) was not observed, t...
متن کاملImmunological distinction between fraction I protein and protochlorophyllide holochrome.
Ribulose 1,5-diphosphate carboxylase (RuDP carboxylase) has been shown to be the major component of the Fraction I protein of leaves (5, 6). Protochlorophyllide associated with a protein, i.e. protochlorophyllide holochrome (PCH), has been isolated from etiolated bean leaves (1, 9,10); upon illumination in zirvo or in vitro it is transformed into chlorophyllide holochrome. Several investigators...
متن کاملEnergy Transfer from NADPH to Protochlorophyllide in Isolated Protochlorophyllide Holochrome as Determined by UV-Fluorescence Excitation Spectroscopy at 77 K
The low -tem perature fluorescence excitation analysis o f different pro tochlorophyllide (PChlide) form s has been extended to the UV part o f the spectrum . A new band at abou t 360 nm was detected in excitation spectra o f active PChlide forms bound to isolated p ro to chlorophyllide holochrom e. This band is very similar to the absorbance o f N A D P H in this region and its intensity dep...
متن کاملMonogalactosyldiacylglycerol Facilitates Synthesis of Photoactive Protochlorophyllide in Etioplasts.
Cotyledon cells of dark-germinated angiosperms develop etioplasts that are plastids containing unique internal membranes called prolamellar bodies (PLBs). Protochlorophyllide (Pchlide), a precursor of chlorophyll, accumulates in PLBs and forms a ternary complex with NADPH and light-dependent NADPH:protochlorophyllide oxidoreductase (LPOR), which allows for the rapid formation of chlorophyll aft...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1971
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.47.5.685